Camels and Camelids

CHARACTERISATION OF SALIVARY GLAND PROTEINS AND P-18 GENE OF CAMEL TICKS FROM BIKANER

Journal Edition: December 2022
Article DOI: 10.5958/2277-8934.2022.00051.0
Published On: 22-12-2022 07:09

The main objective of this study was to investigate the various salivary gland proteins and gene analysis of camel ticks Hyalomma dromedarii found on one-humped camels (Camelus dromedarius) in Bikaner city, Rajasthan, India. For this purpose, Salivary glands were collected from 5 adult ticks (engorged females). Various protein analysis from salivary gland using SDS-PAGE for determination of protein bands and their molecular weights for each fraction was done. The molecular weight of resolved major bands ranged from 14.4 to 96.0 KDa. Out of them large majority of protein molecular weight was around 18 KDa. This low molecular weight band of around 18 KDa was detected in the all 5 fractions of salivary gland of Hy. dromedarii ticks. The protein gene P–18 of Hy. dromedarii of present study had 90.4% sequence identity with that of Hy. asiaticum from China.
Key words: Camel Hyalomma dromedarii, P-18 Gene salivary gland proteins